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BT-474 Clone 5 is an epithelial-like cell line clone that was originally isolated from the mammary gland of a 60-year-old, White female patient with ductal carcinoma. BT-474 (ATCC HTB-20) cells were grown in 10ug/mL Herceptin for several weeks until the resulting cells (clones) were defined as resistant to Herceptin treatment. These resistant cells were grown in standard RPMI with 10% fetal bovine serum for many years under different conditions but the cells enclosed have not been exposed to Herceptin after they were first treated. They were not grown with Herceptin to make sure they stayed resistant. However, these cells have remained resistant for 30+ passages and they still retain the overexpression of HER-2 and are resistant to growth inhibition due to Herceptin.
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NCI-H1618 [H1618] is a cell line isolated from the lungs of a 55-year-old, White, female patient with stage E small cell lung cancer: carcinoma. This product has applications in cancer research.
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Quantitative genomic DNA from Cereibacter sphaeroides strain ATH 2.4.1 can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine bacterial load.
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NCI-H2066 [H2066] is a cell line exhibiting epithelial morphology that was isolated from the lungs of a 70-year-old, White, female patient with stage 1 small cell lung cancer: adenocarcinoma. This product has applications in cancer research.
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Chlamydophila pneumoniae strain AR-39 was isolated from the throat of an infected human with acute pharyngitis in Seattle, Washington. This bacterial strain is propagated in HEp-2 cells (ATCC CCL-23) and has applications in respiratory disease research.
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Escherichia coli strain H10407 was isolated from feces and can be used in the detection of LT toxin and Vibrio cholerae ctx A11. This strain produces heat-labile and heat-stable enterotoxins.
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Chlamydophila DNA, in total DNA, was isolated from Hep-2 cells (ATCC CCL-23) infected with Chlamydophila pneumoniae strain CM-1 (ATCC VR-1360). This product was prepared using methods known to inactivate the infecting agent. It is suitable for use in PCR and other molecular biology applications. The source organism and host cells are also available through the ATCC Catalog.
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